BIOC3390 Tutorials: 16 & 18 November 1999

ATP synthase in cardiac muscle

This topic relies in part on Professor Henderson's bioenergetics lectures to be given in early November 1999. In addition, you may find some useful background material on mitochondrial metabolism and chemical thermodynamics in the Bioenergetics website.

This is the X-ray structure of the beef heart F1 ATPase complexed with efrapeptin, solved by John Walker's team in Cambridge. [Brookhaven code 1EFR.]

Switch to ribbon view and colour in the three alpha and three beta subunits. Click HERE for a brief reminder of the main CHIME commands, or HERE for a full tutorial. Rotate the molecule to display the central camshaft that couples the F0 proton turbines in the inner mitochondrial membrane to the F1 head groups of the ATP synthase exposed in the matrix space.

We would like you to calculate the torque and the angular velocity of the rotating polypeptide camshaft.

Using the figures from the "general introduction", how many ATP synthase molecules are required per g dry weight of heart to account for the observed rates of oxygen consumption and ATP synthesis?

How does your calculated value for the number of ATP synthase molecules compare with the experimental results?

New 19 November 1999 DOWNLOAD a copy of our calculations for this problem.

Abrahams et al (1996) The structure of bovine F1-ATPase complexed with the peptide antibiotic efrapeptin. Proc. Natl. Acad. Sci. USA 93, 9420. [Click HERE for the abstract, or HERE for the full text PDF version.]

Dimroth, P, Kaim, G & Matthey, U (1998) The motor of the ATP synthase BBA-Bioenergetics 1365(1-2), 87-92. [This paper proposes an electrostatic mechanism for the bacterial F0 motor, driven by the sodium ion gradient. No electronic copies are available.]

Dmitriev, OY et al (1999) Structure of the subunit c oligomer in the F1F0 ATP synthase: Model derived from solution structure of the monomer and cross- linking in the native enzyme. Proc. Natl. Acad. Sci. USA 96(14), 7785-7790. [Click HERE for the HTML version, or HERE for PDF format.]

Elston, T et al (1998) Energy transduction in ATP synthase. Nature 391(6666), 510-513. [Write down the volume and page numbers for future reference. Click HERE for access instructions. Click HERE to gain access via the Leeds University network.]

Engelbrecht, S & Junge, W (1997) ATP synthase: A tentative structural model. FEBS Letters 414(3), 485-491. [This paper proposes a model for the holoenzyme, based on the partial structures then published. No electronic copies are available.]

Etzold, C et al (1997) Turnover number of Escherichia coli F0F1 ATP synthase for ATP synthesis in membrane vesicles. Europ. J. Biochem. 243(1-2), 336-343. [Compare these rates with mammalian cells. No electronic copies are available.]

Fillingame, RH (1997) Coupling H+ transport and ATP synthesis ln F1F0-ATP synthases: Glimpses of interacting parts in a dynamic molecular machine. J. Exp. Biol. 200(2), 217-224. [Click HERE for the abstract, which includes a link to a full-text PDF file.]

Fillingame, RH et al (1998) Subunit organization and structure in the F-0 sector of Escherichia coli F1F0 ATP synthase. BBA-Bioenergetics 1365(1-2), 135-142. [Review of cross-linking studies on the F0 basepiece. No electronic copies are available.]

Girvin, ME et al (1998) Solution structure of the transmembrane H+-transporting subunit c of the F1F0 ATP synthase. Biochemistry 37(25), 8817-8824. [Click HERE for the HTML version, or HERE for PDF format.]

Groth, G et al (1998) Molecular architecture of the c-subunit oligomer in the membrane domain of F-ATPases probed by tryptophan substitution mutagenesis. J. Mol. Biol. 281(1), 49-59. [Click HERE for HTML format, or HERE for the PDF version.]

Groth, G & Strotmann, H (1999) New results about structure, function and regulation of the chloroplast ATP synthase (CF0CF1). Physiologia Plantarum, 106(1), 142-148. [No electronic copies are available.]

Haraux, F & deKouchkovsky, Y (1998) Energy coupling and ATP synthase. Photosynthesis Research 57(3), 231-251. [No electronic copies are available.]

Jiang, WP & Fillingame, RH (1998) Interacting helical faces of subunits a and c in the F1F0 ATP synthase of Escherichia coli defined by disulfide cross-linking. Proc. Natl. Acad. Sci. USA 95(12), 6607-6612. [Click HERE for the HTML version, or HERE for PDF format.]

Kagawa, Y (1999) Biophysical studies on ATP synthase. Advances in Biophysics 36, 1-25. [No electronic copies are available.]

Kaim, G & Dimroth, P (1998) ATP synthesis by the F1F0 ATP synthase of Escherichia coli is obligatorily dependent on the electric potential. FEBS Letters 434(1-2), 57-60. [ATP synthase kinetics at different membrane potentials. No electronic copies are available.]

Karrasch, S & Walker, JE (1999) Novel Features in the Structure of Bovine ATP Synthase. J. Mol. Biol. 290(2), 379-384. [Click HERE for HTML format, or HERE for the PDF version.]

Nakamoto, RK et al (1999) Rotational coupling in the F0F1 ATP synthase. Ann. Rev. Biophys. & Biomolec. Struct. 28, 205-234. [Click HERE for the HTML version, or HERE for PDF format.]

Noji, H et al (1997) Direct observation of the rotation of F-1-ATPase. Nature 386, 299-302. [This is the paper mentioned in the "general introduction". Write down the volume and page numbers for future reference. Click HERE for access instructions. Click HERE to gain access via the Leeds University network.]

Rigoulet, M et al (1998) Quantitative analysis of some mechanisms affecting the yield of oxidative phosphorylation: Dependence upon both fluxes and forces. Molecular & Cellular Biochemistry 184(1-2), 35-52. [This issue appears to have been a Rigoulet special event, since it contains six(!) other papers by the same author. Unforunately, we no longer subscribe to this journal in Leeds, so electronic copies are not available. These authors believe that the respiratory chain and the ATP synthase have variable proton stoichiometries. Read the abstracts of this paper, and the following paper, via BIDS, since they could be important.]

Sabbert, D & Junge, W (1997) Stepped versus continuous rotatory motors at the molecular scale. Proc. Nat. Acad. Sci. USA 94(6), 2312-2317. [Click HERE for the HTML version, or HERE for PDF format.]

Sabbert, D et al (1997) Functional and idling rotatory motion within F1-ATPase. Proc. Nat. Acad. Sci. USA 94, 4401-4405. [Click HERE for the HTML version, or HERE for PDF format.]

Wang, HY & Oster, G (1998) Energy transduction in the F-1 motor of ATP synthase. Nature 396(6708), 279-282. [Write down the volume and page numbers for future reference. Click HERE for access instructions. Click HERE to gain access via the Leeds University network.]

Wilkens, S & Capaldi, RA (1998) Electron microscopic evidence of two stalks linking the F-1 and F-0 parts of the Escherichia coli ATP synthase. BBA-Bioenergetics 1365(1-2),93-97. [No electronic copies are available.]

Wilkens, S & Capaldi, RA (1998) ATP synthase's second stalk comes into focus. Nature 393(6680), 29. [Write down the volume and page numbers for future reference. Click HERE for access instructions. Click HERE to gain access via the Leeds University network.]

Most electronic journals check your browser IP address and require an institutional subscription to the printed version of the journal. Consequently, many of the hypertext links in this reference list will only work from a university campus computer.



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