www.plantprobes.net

 Molecular probes for plant cell wall polymers

PlantProbes is a distribution service for monoclonal antibodies and molecular probes directed to plant cell wall components generated by the Paul Knox Cell Wall Lab at the University of Leeds. Funds received are used to maintain antibody stocks and antibody availability.

Contact & Ordering Information
 PlantProbes is based in Leeds in the UK. All enquiries & requests for monoclonal antibodies should be by email or fax. Please note that PlantProbes runs a reduced service during July and August. To order please let us know the items required, delivery and invoice addresses and a purchase order number if appropriate. Items can be paid for by institutional invoice, credit card or PayPal - ask for details.

 
Email: antibodies@plantprobes.net      FAX: +44-(0)113-3433144
 

October 2009: New products and pricing scheme

Rat monoclonal antibodies can be supplied as 1 ml or 5 ml units of hybridoma cell culture supernatants. Select any combination of rat monoclonal antibodies. Costs in UK£ (GBP) are as follows:

# units

1 ml

5 ml

1

100

250

2

200

450

3

270

630

4

350

770

5

400

870

 

 

 


 

Download the new PlantProbes catalogue.pdf      

 JIM- rat monoclonal antibodies to AGPs and extensins.... click here

LINKS to related sites.... click here

PlantProbes rat hybridoma cell culture supernatants contain 0.1% sodium azide as a preservative. They are shipped at ambient temperature and should be stored at 4oC.

PlantProbes products:

____

RAT hybridoma cell culture supernatants

Hemicellulose probes

LM10   Anti-xylan

LM11   Anti-xylan / arabinoxylan  

LM15   Anti-xyloglucan  


Pectin probes

LM7     Anti-homogalacturonan

LM18   Anti-homogalacturonan

LM19   Anti-homogalacturonan

LM20   Anti-homogalacturonan

JIM5   Anti-homogalacturonan

JIM7   Anti-homogalacturonan

LM8   Anti-xylogalacturonan  

LM5   Anti-(1-4)-b-D-galactan

LM9   Anti-feruloylated-galactan

LM6   Anti-(1-5)-a-L-arabinan

LM13   Anti-(1-5)-a-L-arabinan (linear)

LM16   Anti-processed arabinan /RG-I


Glycoprotein probes

LM2   Anti-arabinogalactan-protein

LM14   Anti-arabinogalactan-protein

LM1  Anti-extensin 

____

HIS-tagged recombinant proteins

PAM1  Anti-homogalacturonan scFv

CBM3a Crystalline cellulose-directed CBM

____

MOUSE hybridoma cell culture supernatant

2F4   Anti-homogalacturonan

   
 

Details of antibodies/molecular probes:

______________

Rat monoclonal antibody cell culture supernatants
 

Cat. No. LM10

MONOCLONAL ANTIBODY to XYLAN / LM10

(Rat IgG2c)

SPECIFICITY: Generated using a neoglycoprotein (xylopentaose-BSA). It can recognise unsubstituted and relatively low-substituted xylans in several species. It has no cross-reactivity with wheat arabinoxylan.

REFERENCE: McCartney et al. (2005) J. Histochem. Cytochem 53, 543-546. ONLINE

 

Cat. No. LM11

MONOCLONAL ANTIBODY to XYLAN / ARABINOXYLAN / LM11

(Rat IgM)

SPECIFICITY: Generated using a neoglycoprotein (xylopentaose-BSA). It can recognise unsubstituted and relatively low-substituted xylans in several species. It can also accommodate more extensive substitution of a xylan backbone and binds strongly to wheat arabinoxylan. 

REFERENCE: McCartney et al. (2005) J. Histochem. Cytochem 53, 543-546. ONLINE

 

Cat. No. LM15

MONOCLONAL ANTIBODY to XYLOGLUCAN / LM15 

(Rat IgG2c)

SPECIFICITY: Generated using a neoglycoprotein incorporating the xylosylated heptasaccharide from tamarind xyloglucan (XXXG-BSA). It recognises the XXXG motif of xyloglucan in several species and can accommodate to some extent a single galactosyl residue. 

REFERENCE: Marcus et al. (2008) BMC Plant Biology 8, 60. ONLINE

 

Cat. No. LM7

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / LM7

(Rat IgM)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody recognises a partially methyl-esterified epitope of homogalacturonan that results from non-blockwise de-esterification processes. It does not bind to un-esterified homogalacturonan.

REFERENCES: Willats et al. (2001) J. Biol. Chem 276, 19404-19413 ONLINE; Clausen et al. (2003) Carbohydr. Res. 338, 1797-1800. ONLINE

 

Cat. No. LM18

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / LM18

(Rat IgG2c)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody has some preference for partially methyl-esterified homogalacturonan but can also bind to un-esterified homogalacturonan.

REFERENCES: Verhertbruggen et al. (2009) Carbohydr. Res. 344, 1858-1862. ONLINE

 

Cat. No. LM19

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / LM19

(Rat IgM)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody recognises a range of homogalacturonan samples but appears to have a preference for and binds strongly to un-esterified homogalacturonan. We now recommend the use of LM19 in the place of JIM5 as LM19 binds more effectively to unesterified HG.

REFERENCES: Verhertbruggen et al. (2009) Carbohydr. Res. 344, 1858-1862. ONLINE

 

Cat. No. LM20

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / LM20

(Rat IgM)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody requires methyl-esters for recognition of homogalacturonan and does not bind to un-esterified homogalacturonan. We now recommend the use of LM20 in the place of JIM7 as LM20 binding is more effectively lost by high pH treatments that remove HG methyl esters.

REFERENCES:  Verhertbruggen et al. (2009) Carbohydr. Res. 344, 1858-1862. ONLINE

 

Cat. No. JIM5

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / JIM5

(Rat IgG, JIM5)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody recognises partially methyl-esterified epitopes of homogalacturonan and can also bind to un-esterified homogalacturonan.

We now recommend the use of LM19 in the place of JIM5 as LM19 binds more effectively to unesterified HG.

REFERENCES: Knox et al. (1990) Planta 181, 512-521; Willats et al. (2000) Carbohydr.  Res. 327, 309-320; Clausen et al. (2003) Carbohydr. Res. 338, 1797-1800 ONLINE; Verhertbruggen et al. (2009) Carbohydr. Res. 344, 1858-1862. ONLINE

 

Cat. No. JIM7

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / JIM7

(Rat IgA, JIM7)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody recognises partially methyl-esterified epitopes of homogalacturonan but does not bind to un-esterified homogalacturonan.

We now recommend the use of LM20 in the place of JIM7 as LM20 binding is more effectively lost by high pH treatments that remove HG methyl esters.

REFERENCES: Knox et al. (1990) Planta 181, 512-521; Willats et al. (2000) Carbohydr.  Res. 327, 309-320; Clausen et al. (2003) Carbohydr. Res.  338, 1797-180 ONLINE; Verhertbruggen et al. (2009) Carbohydr. Res. 344, 1858-1862. ONLINE

    

Cat. No. LM8

MONOCLONAL ANTIBODY to XYLOGALACTURONAN / LM8

(Rat IgM, LM8)

SPECIFICITY: The antibody recognises a specific epitope of a xylogalacturonan pectic polysaccharide that is associated with cell detachment and separation in a wide range of species. It has no known cross-reactivity with other polymers. This antibody does NOT bind to all xylogalacturonans.

REFERENCE: Willats et al. (2004) Planta  218, 673-681. ONLINE

 

Cat. No. LM5

MONOCLONAL ANTIBODY to (1-4)-β-D-GALACTAN  / LM5

(Rat IgG)

SPECIFICITY: The antibody was generated using a neoglycoprotein (galactotetraose-BSA). It recognises a linear tetrasaccharide in (1-4)-β-D-galactans. It has no cross-reactivity with (1-3)-β-D-galactans or (1-6)-β-D-galactans. It can recognise pectic polysaccharides in several species. In competitive inhibition ELISA, antibody binding to (1-4)-β-D-galactan was inhibited (50%) by 58 µg/ml (1-4)-β-D-galactotetraose and by 0.7 µg/ml lupin (1-4)-β-D-galactan.

REFERENCE: Jones et al. (1997) Plant Physiol. 113, 1405-1412. ONLINE

 

Cat. No. LM9

MONOCLONAL ANTIBODY to FERULOYLATED-(1-4)-β-D-GALACTAN

(Rat IgM)

SPECIFICITY: The antibody recognises a specific epitope of a feruloylated-(1-4)-b-D-galactan that is a structural feature of the pectic polymers of plant species of the Amaranthaceae/Chenopodiaceae. It has no known cross-reactivity with other polymers. In competitive inhibition ELISAs, antibody binding to sugar beet pectin was inhibited (50%) by 15 μg/ml O-[6-O-(trans-feruloyl)-b-d-galactopyranosyl]-(1→4)-d-galactopyranose.

REFERENCE: Clausen et al. (2004) Planta  219, 1036-1041. ONLINE

 

Cat. No. LM6

MONOCLONAL ANTIBODY to (1-5)-a-L-ARABINAN / LM6

(Rat IgG)

SPECIFICITY: Generated using a neoglycoprotein (arabinoheptaose-BSA). Recognises a linear pentasaccharide in (1-5)-α-L-arabinans. It can recognise pectic polysaccharides in several species. It has no cross-reactivity with gum arabic but it may recognize arabinogalactan-proteins (AGPs) in some species. In competitive inhibition ELISAs, antibody binding to (1-5)-α-L-arabinan was inhibited (50%) by 40 ng/ml (1-5)-α-L-arabinopentaose and 19 ng/ml (1-5)-α-L-arabino-hexaose.  

REFS: Willats et al. (1998) Carbohydr. Res. 308, 149-152 ONLINE; Verhertbruggen et al. (2009) Plant J. 59, 413-425. ONLINE

 

Cat. No. LM13

MONOCLONAL ANTIBODY to (1-5)-a-L-ARABINAN (linear) / LM13

(Rat IgG)

SPECIFICITY: Isolated from a HTP screen of antibodies generated subsequent to immunization with a pectic fraction. Recognises a linear epitope in (1-5)-α-L-arabinans. Antibody recognition of arabinans increases with arabinofuranosidase action. This antibody binds to a specific subset of pectic arabinans, and to longer stretches of 1,5-linked arabinosyl residues that are likely to be more abundant in unbranched arabinans. The binding of LM13 is more sensitive to arabinanase action than LM6.

REFS: Moller et al. (2007) Glycoconjugate J. 25, 37-48; Verhertbruggen et al. (2009) Plant J. 59, 413-425. ONLINE

 

Cat. No. LM16

MONOCLONAL ANTIBODY to PROCESSED ARABINAN - RG-I / LM16

(Rat IgM)

SPECIFICITY: Generated subsequent to immunization with a pectic fraction. Recognises a epitope associated with arabinans and can be generated by arabinofuranosidase action and the loss of arabinosyl residues. The binding of LM16 is sensitive to galactosidase action and the epitope may involve galactosyl residue(s) on RG backbones.  

REFERENCE: Verhertbruggen et al. (2009) Plant J. 59, 413-425. ONLINE

 

Cat. No. LM2

MONOCLONAL ANTIBODY to ARABINOGALACTAN-PROTEIN  / LM2

(Rat IgM)

SPECIFICITY: Generated to rice arabinogalactan-proteins (AGPs). Recognises a carbohydrate epitope containing b-linked glucuronic acid. It can recognise AGPs in several species. In competitive inhibition ELISAs antibody binding to gum arabic was inhibited (50%) by 70 mg/ml 1-O-methyl-b-D-GlcA.  The binding of the antibody to AGPs can be fully inhibited by 10 mM 1-O-methyl-b-D-GlcA.

REFERENCES: Smallwood et al. (1996) Planta 198, 452-459; Yates et al. (1996) Glycobiology 6, 131-139. ONLINE

 

Cat. No. LM14

MONOCLONAL ANTIBODY to ARABINOGALACTAN-PROTEIN  / LM14

(Rat IgM)

SPECIFICITY: Isolated from a HTP screen of antibodies generated subsequent to immunization with a pectic fraction. Recognizes arabinogalactan-proteins and will also bind to larch arabinogalactan. It can recognise AGPs in several species.

REFERENCE: Moller et al. (2007) Glycoconjugate J. 25, 37-48. ONLINE

 

Cat. No. LM1

MONOCLONAL ANTIBODY to EXTENSIN / LM1

(Rat IgM)

SPECIFICITY: Generated to rice extensin hydroxyproline-rich glycoproteins (HRGPs). The antibody recognises an epitope that is carried by a range of HRGPs of the extensin class in a wide range of angiosperm species. The LM1 epitope most likely includes extensin glycan components.

REFERENCE: Smallwood et al. (1995) Planta 196, 510-522.

 

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His-tagged recombinant proteins

 

Cat. No. PAM1

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / PAM1

(Phage display-derived scFv)

SPECIFICITY: The antibody recognises the homogalacturonan domain of pectic polysaccharides. It has no known cross-reactivity with other polymers. It can recognise pectic polysaccharides in several species. The antibody is specific to unesterified homogalacturonan and recognises long un-esterified blocks of GalA residues.

REFERENCES: Willats et al. (1999) Plant Journal 18, 57-65; Willats et al. (2000) Carbohydrate Research 327, 309-320; Manfield et al. (2005) Plant Science 169, 1090-1095.

SUPPLY: PAM1 is provided as a his-tagged recombinant soluble scFv. PAM1 DATA SHEET

COST: £150 for 50 μg his-tagged PAM1 scFv.

Cat. No. CBM3a

CRYSTALLINE CELLULOSE-BINDING MODULE / CBM3a

(Recombinant CBM protein)

SPECIFICITY: This type A carbohydrate-binding module (CBM) from Clostridium thermocellum is directed to crystalline cellulose and can detect cellulose in both in vitro assays and directly in plant materials.

REFERENCES: Blake et al. (2006) J Biol. Chem. 281, 29321-29329. ONLINE

SUPPLY: CBM3a is provided as a his-tagged recombinant protein.

COST: £200 for 200 μg his-tagged CBM3a protein.

______________

Mouse monoclonal antibody cell culture supernatant

 

Cat. No. 2F4

MONOCLONAL ANTIBODY to HOMOGALACTURONAN / 2F4  

(Mouse IgG1)

SPECIFICITY: The 2F4 mouse monoclonal antibody recognises the homogalacturonan domain of pectic polysaccharides. It binds specifically to a dimeric association of pectic chains through calcium ions. It binds to homogalacturonans with degrees of methyl-esterification (DM) up to 40%.

REFERENCES: Liners et al. (1989) Plant Physiology 91, 1419-1424; Liners et al. (1992) Plant Physiology 99, 1099-1104; Liners & van Cutsem (1992) Protoplasma 170, 10-21.

SUPPLY: 2F4 is provided as a 1 mL volume of mouse hybridoma supernatant. 2F4 DATA SHEET

COST: £175 per 1 mL unit of 2F4 hybridoma supernatant.

 

 

 

 

   
 

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Last modified: 09/10/2009